flag peptide molecular weight Molecular Weight: 2,861.87 Da

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Dr. Andrew Parker

flag peptide molecular weight 1012.96 Da - what-are-peptides-for-weight-loss-for-females Molecular Weight: 2,861.87 Da Understanding the Molecular Weight of FLAG Peptides

what-are-peptides-in-moisturizer The FLAG peptide, often recognized by its sequence DYKDDDDK, is a widely utilized epitope tag in molecular biology and biochemistry. Understanding its molecular weight is crucial for various applications, including protein purification, detection, and analysis.Thepeptidesequence of theFLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). Additionally,FLAG-tags may be used in tandem, commonly the ... Extensive research and data from commercial suppliers consistently place the molecular weight of the standard FLAG peptide around 1012.97 Daltons (Da), with slight variations such as 1013.0 g/mol, 1012.96 Da, 1012.98, and 1012.99. These values are derived from its molecular formula, typically reported as C41H60N10O20. The peptide nature of this sequence, composed of specific amino acids, dictates its precise mass.

Entity: FLAG peptide

LSI: Molecular Weight, peptide, DYKDDDDK

Variation: 1012.97, 1013Flag-tag and 3x Flag-tag.0 g/mol, 1012.96 Da, 1012.98, 1012.99, C41H60N10O20, FLAG

The Significance of Molecular Weight in FLAG Peptide Applications

The precise molecular weight of the FLAG peptide is a fundamental parameter that informs its behavior and utility.Chemical Properties of FLAG tag Peptide ; M.Wt,1012.97; Solubility, ≥50.6 mg/mL in DMSO, ≥210.6 mg/mL in Water, ≥34.03 mg/mL in EtOH ; Storage, Store at -20°C, ... For instance, in Western blotting or ELISA, knowing the molecular weight of the tag helps in identifying the presence and size of the tagged protein.3X Flag-tag Peptide -20571ES - Yeasen When designing purification strategies using anti-FLAG antibodies for FLAG tag protein purification, the affinity of the antibody to the FLAG epitope is paramount, and the consistent molecular weight reinforces the defined nature of this epitopeTriple FLAG® Peptide (000-000-H47).

Entity: FLAG tag protein purification, FLAG

LSI: molecular weight, FLAG peptide

Variation: FLAG tag

Variations in FLAG Peptide Constructs and Their Molecular Weights

While the single FLAG peptide (DYKDDDDK) has a well-established molecular weight of approximately 1012.97 Da, researchers also employ multi-copy FLAG tags, such as 3x FLAG peptide.Empirical Formula (Hill Notation):. C41H60N10O ; Molecular Weight:1012.97; UNSPSC Code: 12352202 ; PubChem Substance ID: 329799698 ; NACRES: NA.32. These constructs, designed for enhanced detection or binding, naturally have significantly higher molecular weights. For example, a 3x FLAG peptide is reported to have a molecular weight of 2,861Molecular Weight ·1012.96 Da; Source · Synthetic ; Format · Lyophilized white powder ; Storage (short term): 2-8°C (up to 6 months) ; Storage (long term): -20°C.87 Da or 2975.84. These larger constructs are often needed in specific experimental setups.Purity:99.87% ; Chemical Properties.Molecular Weight, 3002.88. Formula, C ; Storage, keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for ... The molecular formula for a 3x FLAG peptide can be considerably more complex, such as C120H169N31O49S. In some instances, longer versions like the Triple FLAG® Peptide are synthesized, consisting of 23 amino acid residues and a molecular weight of 2861.9 daltons. The existence of these variations, including constructs with different molecular weights like 1203DYKDDDDK Peptide.31 and 111Flag-Tag | Definition & Data.3 kDa (observed in a recombinant protein with an N-terminal 6xHis and Flag tag), highlights the adaptability of the FLAG tag system.

Entity: 3x FLAG peptide, Triple FLAG® Peptide

LSI: FLAG peptide, molecular weight, FLAG

Variation: 2,861.87 Da, 2975Product Quantity. 5 mg, 100 mg, 1 gram ; Purity. >98% ; Catalog Number. LT12022 ; Molecular Weight.1013; Formula. DYKDDDDK..84, C120H169N31O49S, 111The Triple FLAG® Peptide is a synthetic peptide of 23 amino acid residues (molecular weight of 2861.9 daltons). The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated ....3 kDa, 6xHis and Flag tag, 1203.31

Reagent Properties and Handling of FLAG Peptides

Beyond its molecular weight, other properties of the FLAG peptide are important for its effective useRecombinant TET3 (824-1795) protein. These peptides are typically supplied as lyophilized white powderChemical Information, Storage & Stability. Molecular Weight,1012.97, Formula. C41H60N10O20.. Their solubility is often mentioned, with FLAG peptide being soluble in water or 1% acetic acid, and also soluble in DMSO or ethanol to varying degrees.Molecular Weight.2861.87. Formula. C120H169N31O49S. Purity. 99.35. Solubility. ≥143.1mg/mL in DMSO, <14.35mg/mL in EtOH, ≥143.4mg/mL in H2O with gentle warming ... For storage, short-term recommendations usually involve refrigeration at 2-8°C for up to six months, while long-term storage is typically at -20°C.3× FLAG peptide | Protein purification The purity of commercially available FLAG peptides is generally high, often exceeding 98% or even 99.69%. These characteristics contribute to the reliability and reproducibility of experiments employing the FLAG tag, whether it’s for FLAG tag applications or peptide based researchMolecular Weight: 2,861.87 Da. Quantity: 5 mg. N-terminus: NH2. C-terminus: COOH. £170.89. Add to cart. Additional details. UOM, 5 mg. 3x FLAG peptide (5 mg) .... The IUPAC nomenclature and details found on platforms like PubChem provide comprehensive information on these reagents3X Flag-tag Peptide -20571ES - Yeasen.

Entity: FLAG peptide, FLAG tag

LSI: peptide, molecular weight, FLAG

Variation: 1% acetic acid, 98%, 99.Molecular Formula, C120H169N31O49S ;Molecular Weight, 2861.87 ; Appearance, White Powder ; Purity, ≥98% ; Solubility, Soluble in water (10mM) or 1% acetic acid ...69%, IUPAC

In summary, the FLAG peptide, with its consistent molecular weight around 1012.97 Da, serves as a vital tool in biological research. Understanding this and other associated properties, including the molecular weights of multi-copy tags and various reagent specifications, is essential for successful experimental design and execution. The availability of detailed information, including molecular formula and solubility, underscores the well-characterized nature of this important peptide tag.

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